Chemical speciation of MeHg+ and Hg2+ in aqueous solution and HEK cells nuclei by means of DNA interacting fluorogenic probes† †Electronic supplementary information (ESI) available: Experimental details, characterization data, and additional experiments. See DOI: 10.1039/c5sc00718f

نویسندگان

  • Borja Díaz de Greñu
  • José García-Calvo
  • José Cuevas
  • Gabriel García-Herbosa
  • Begoña García
  • Natalia Busto
  • Saturnino Ibeas
  • Tomás Torroba
  • Blanca Torroba
  • Antonio Herrera
  • Sebastian Pons
چکیده

Selected new fluorogenic probes that interact in different ways with Hg and MeHg have been prepared and used for the chemical speciation of both cations in aqueous solution as well as in HEK293 cells. The best selective speciation of Hg and MeHg has been achieved by in vitro approaches based on fluorogenic probes supported in cultured cells, due to the particular sensitivity of the HEK293 cells to permeation by Hg, MeHg and the fluorogenic probes. In particular, MeHg was selectively detected in cell nuclei by probe JG45.

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منابع مشابه

Green- to far-red-emitting fluorogenic tetrazine probes – synthetic access and no-wash protein imaging inside living cells† †Electronic supplementary information (ESI) available: Synthetic procedures and spectroscopic data, details of cell experiments and imaging. See DOI: 10.1039/c6sc03879d Click here for additional data file.

Fluorogenic probes for bioorthogonal labeling chemistry are highly beneficial to reduce background signal in fluorescence microscopy imaging. 1,2,4,5-Tetrazines are known substrates for the bioorthogonal inverse electron demand Diels–Alder reaction (DAinv) and tetrazine substituted fluorophores can exhibit fluorogenic properties. Herein, we report the synthesis of a palette of novel fluorogenic...

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عنوان ژورنال:

دوره 6  شماره 

صفحات  -

تاریخ انتشار 2015